Nonenzymatic labeling of 5-hydroxymethylcytosine in nanopore sequencing.
نویسندگان
چکیده
Labeling chemically for a "single" result: A one-step, nonenzymatic 5hmC thiolation method mediated by bisulfite is highlighted. The 5hmC base can be chemically labeled in single-stranded DNA and detected by using nanopore sequencing.
منابع مشابه
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عنوان ژورنال:
- Chembiochem : a European journal of chemical biology
دوره 14 11 شماره
صفحات -
تاریخ انتشار 2013